Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Goat IgG
View all DR3/TNFRSF25 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DR3/TNFRSF25
Gln31-Phe199
Accession # AAK11256

Specificity

Detects mouse DR3 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) DR6, rmTrail R2, and recombinant human (rh) Mer is observed, and approximately 20% cross-reactivity with rhDR3, rhTrail R4, rmCD30, and rmHVEM is observed, and less than 5% cross-reactivity with rhTrail R3, rm4-1BB, rhTrail R1, rmGITR, and rmTNF R1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for DR3/TNFRSF25 Antibody [CoraFluor™ 1]

DR3/TNFRSF25 Antibody [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for DR3/TNFRSF25 Antibody [CoraFluor™ 1]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: DR3/TNFRSF25

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family, through their death domain containing receptors TNFR1 and Fas. A cell surface receptor with a death domain, DR3, has been identified by several groups independently, although it has been given alternative names. The ligand for DR3 has very recently been identified as TWEAK, also termed Apo3L. DR3 is highly expressed in tissues enriched in lymphocytes including PBL, thymus and spleen. DR3 mediates activation of NF kappa B, and induces apoptosis. DR3 may also play a role in regulating lymphocyte homeostasis.

Long Name

Death Receptor 3

Alternate Names

Apo-3, LARD, TNFRSF25, TRAMP, WSL-1

Gene Symbol

TNFRSF25

Additional DR3/TNFRSF25 Products

Product Documents for DR3/TNFRSF25 Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DR3/TNFRSF25 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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