Drosophila Smad2 Antibody

R&D Systems | Catalog # AF7948

R&D Systems

Key Product Details

Species Reactivity

Validated:

Drosophila

Cited:

Drosophila

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Smad2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Drosophila Smad2
Gly262-Ser486
Accession # O96660

Specificity

Detects Drosophila Smad2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Smad1 is observed, and less than 1% cross-reactivity with rhSmad2, rhSmad3, and rhSmad5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Drosophila Smad2 Antibody

Detection ofDrosophilaSmad2 antibody by Western Blot.

Detection ofDrosophilaSmad2 by Western Blot.

Western blot shows recombinantDrosophilaSmad2 (2 ng/lane). PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Smad2 (C-terminal fragment) at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection ofDrosophilaSmad2 antibody by Western Blot.

Detection ofDrosophilaSmad2 by Western Blot.

Western blot shows wild-type and Smad2 null mutationDrosophilalarval extracts. PVDF membrane was probed with 0.7 µg/mL of Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by Anti-Sheep IgG Secondary Antibody. A specific band was detected for Smad2 at the expected mobility of approximately 58 kDa, but not mutant extracts. This experiment was conducted under reducing conditions. dSmad2 null mutation is described in Peterson, AJ,et al.(2012) PLoS One7: e36548.Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Smad2 inDrosophilaLarvae.

Smad2 inDrosophilaLarvae.

Smad2 was detected in wild-type and Smad2 null mutationDrosophilalarvae using Sheep Anti-DrosophilaSmad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) at 0.7 μg/mL. Tissue was stained using a fluorescent Anti-Sheep IgG Secondary Antibody. A single confocal image in the upper panel depicts detection of endogenous Smad2 in epidermal and central nervous system cells. The dSmad2 mutant embryo in the lower panel was stained and imaged identically and reveals background staining. dSmad2 null mutation is described in Peterson, AJ,et al.(2012) PLoS One 7: e36548.Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.

Applications for Drosophila Smad2 Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-0.7 µg/mL
Sample: Drosophila larvae, wild-type and dSmad2 null mutation

Western Blot

0.1-0.7 µg/mL
Sample: Recombinant Drosophila Smad2 and Drosophila larval extracts

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Smad2

Smad2 (SMAll body plus Mothers Against Decapentaplegic; also SmoX [Smad on Chr X]) is a 54 kDa (predicted) member of the receptor regulated Smad family of proteins. It is a downstream component of the Drosophila activin/TGF beta signaling pathway, and appears to play a key role in cell proliferation. Analogous to the system in vertebrates, stimulation of the fruitfly activin type I receptor (baboon):type II receptor (punt) complex results in dSmad2 (Drosophila Smad2) phosphorylation and subsequent interaction with dSmad4/Medea. As in vertebrates, the Drosophila Samd2/Smad4 complex enters the nucleus and interacts with a transcriptional cofactor(s) termed TGIF. At this point, the vertebrate:invertebrate systems diverge over the nature of the TGIF cofactor(s); vertebrate TGIF is a corepressor, while invertebrate TGIF (dTGIF) is a coactivator. Drosophila Smad2 is 486 amino acids (aa) in length. It contains two Mad homology domains (aa 7-130 and 285-475), the former of which participates in DNA-binding. Over aa 262-486, Drosophila Smad2 shares 70% aa sequence identity with both mouse and human Smad2.

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Entrez Gene IDs

4087 (Human); 17126 (Mouse); 29357 (Rat); 31738 (Drosophila)

Gene Symbol

SMAD2

UniProt

O96660 (Drosophila)

Additional Smad2 Products

Product Documents for Drosophila Smad2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Drosophila Smad2 Antibody

For research use only

Citations for Drosophila Smad2 Antibody

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Protocols

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