ECRG4 Antibody (OTI3B10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72294

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3B10

Format

Azide and BSA Free
View all ECRG4 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human C2orf40 (NP_115787) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ECRG4 Antibody (OTI3B10) - Azide and BSA Free

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]

Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Jurkat cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for ECRG4 Antibody (OTI3B10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ECRG4

ECRG4, also known as C2orf40 or Chromosome 2 Open Reading Frame 40, is a secreted hormone that may stimulate senescence of neural precursor cells and oligodendrocytes. Current research on ECRG4is being performed in relation to several diseases and disorders including esophageal cancer, esophagitis and breast cancer. ECRG4 has also been shown to interact with p53.

Alternate Names

chromosome 2 open reading frame 40, ECRG4augurin, esophageal cancer related gene 4 protein, Esophageal cancer-related gene 4 protein

Gene Symbol

ECRG4

Additional ECRG4 Products

Product Documents for ECRG4 Antibody (OTI3B10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ECRG4 Antibody (OTI3B10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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