eEF1A1 Antibody (24GB3775)

Novus Biologicals | Catalog # NBP3-50742

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB3775
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Product Specifications

Immunogen

A synthesized peptide derived from human eEF1A1 (Uniprot: P68104)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eEF1A1 Antibody (24GB3775)

eEF1A1 Antibody (24GB3775)

Immunocytochemistry/ Immunofluorescence: eEF1A1 Antibody (24GB3775)[NBP3-50742] -

Immunocytochemical staining of HepG2 cells with eEF1A1 antibody (1:1,000). Nuclei were stained blue with DAPI; eEF1A1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
eEF1A1 Antibody (24GB3775)

Flow Cytometry: eEF1A1 Antibody (24GB3775)[NBP3-50742] -

Flow cytometric analysis of eEF1A1expression in HepG2 cells using eEF1A1 antibody (1:2,000). Green, isotype control; red, Eukaryotic translation elongation factor 1 alpha 1.
eEF1A1 Antibody (24GB3775)

Western Blot: eEF1A1 Antibody (24GB3775)[NBP3-50742] -

Western blotting analysis using eEF1A1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for eEF1A1 Antibody (24GB3775)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eEF1A1

The eEF1A1 gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for theenzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung,liver, kidney, and pancre

Alternate Names

CCS3, CCS-3, cervical cancer suppressor 3, CTCL tumor antigen, EE1A1, EEF-1, EEF1A, eEF1A-1, EF1AHNGC:16303, EF1a-like protein, EF-1-alpha-1, EF-Tu, elongation factor 1 alpha subunit, elongation factor 1-alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, eukaryotic translation elongation factor 1 alpha 1, eukaryotic translation elongation factor 1 alpha 1-like 14, FLJ25721, glucocorticoid receptor AF-1 specific elongation factor, GRAF-1EF, LENG7, leukocyte receptor cluster (LRC) member 7, Leukocyte receptor cluster member 7, MGC102687, MGC131894, MGC16224, prostate tumor-inducing protein 1, PTI1, translation elongation factor 1 alpha 1-like 14

Gene Symbol

EEF1A1

Additional eEF1A1 Products

Product Documents for eEF1A1 Antibody (24GB3775)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for eEF1A1 Antibody (24GB3775)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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