EHHADH Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84915

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TSGRRILADEALKLGILDKVVNSDPVEEAIRFAQRVSDQPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACVRAVQAAVQYPYEVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EHHADH Antibody - BSA Free

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915]

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915]

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915] - Staining in human liver and cerebral cortex tissues using anti-EHHADH antibody. Corresponding EHHADH RNA-seq data are presented for the same tissues.
Western Blot: EHHADH Antibody [NBP1-84915]

Western Blot: EHHADH Antibody [NBP1-84915]

Western Blot: EHHADH Antibody [NBP1-84915] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915]

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915]

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915]

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915]

Immunohistochemistry-Paraffin: EHHADH Antibody [NBP1-84915] - Staining of human liver shows high expression.

Applications for EHHADH Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EHHADH

The protein encoded by the EHHADH gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomalbeta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while theC-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomaldisorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for thisgene. (provided by RefSeq)

Alternate Names

enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase, enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase, LBFP, LBP, L-PBE, PBFEMGC120586, peroxisomal, peroxisomal bifunctional enzyme3,2-trans-enoyl-CoA isomerase, peroxisomal enoyl-CoA hydratase

Gene Symbol

EHHADH

Additional EHHADH Products

Product Documents for EHHADH Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EHHADH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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