eIF4A2 Antibody

Novus Biologicals | Catalog # NBP2-24529

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine, Chicken, Primate

Cited:

Human

Predicted:

Bovine (100%), Equine (100%), Monkey (100%), Opossum (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all eIF4A2 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 50-100 of human eIF4A2 was used as the immunogen for the antibody.

Specificity

The immunogen for this antibody has 100% homology to eIF4A1 and 89% homology to eIF4A3. Specificity to eIF4A2 has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4A2 Antibody

Western Blot: eIF4A2 Antibody [NBP2-24529]

Western Blot: eIF4A2 Antibody [NBP2-24529]

Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.
Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529]

Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529]

Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: eIF4A2 Antibody [NBP2-24529]

Simple Western: eIF4A2 Antibody [NBP2-24529]

Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for eIF4A2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml

Simple Western

1:200

Western Blot

0.025-0.05 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200

Reviewed Applications

Read 1 review rated 5 using NBP2-24529 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF4A2

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Alternate Names

ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2

Gene Symbol

EIF4A2

UniProt

Q14240 (Human)

Additional eIF4A2 Products

Product Documents for eIF4A2 Antibody

Certificate of Analysis

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Product Specific Notices for eIF4A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for eIF4A2 Antibody

Customer Reviews for eIF4A2 Antibody (1)

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  • eIF4A2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse Embryonic Stem Cells
    Species: Mouse
    Verified Customer | Posted 02/18/2019
    ~20 ug of lysate was separated on SDS-PAGE, transferred to a PVDF membrane, blocked in 5% milk. Incubated overnight with 1:1000 dilution of eIFA2 antibody. Incubated 1:10,000 with secondary anti-rabbit and visualized on a ChemiDoc using ECL.
    eIF4A2 Antibody NBP2-24529

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