EML4 Antibody

Name: EML4 Antibody
Brand: Novus Biologicals
SKU: NBP1-05973
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-05973

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all EML4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human echinoderm microtubule associated protein like 4 using the numbering given in entry NP_061936.2 (GeneID 27436).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EML4 Antibody

Western Blot: EML4 Antibody [NBP1-05973]

Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973]

Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EML4 used at a dilution of1:1000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: EML4 Antibody [NBP1-05973]

Immunoprecipitation: EML4 Antibody [NBP1-05973] - Detection of human EML4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EML4 antibody NBP1-05973 (lot NBP1-05973-2) used for IP at 6 ug per reaction. EML4 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05973-1) and rabbit anti-EML4 antibody NBP1-05974. For blotting immunoprecipitated EML4, NBP1-05973 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for EML4 Antibody

Application

Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000

Application Notes

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EML4

Echinoderm microtubule associated protein like 4 (EML4) has been identified as part of a gene fusion with the kinase domain of the anaplastic lymphoma receptor tyrosine kinase (ALK) in non-small cell lung cancer. EML4 associates with in vitro polymerized microtubules. In the cell, phosphorylated EML4 associates with the mitotic spindle and is essential for proliferation and microtubule network formation. Alternative names for EML4 include EMAPL4, ropp120, C2orf2, ELP120, and EMAP-4.

Alternate Names

C2orf2, echinoderm microtubule associated protein like 4, echinoderm microtubule-associated protein-like 4, ELP120, EMAP-4, EMAPL4, FLJ10942, FLJ32318, Restrictedly overexpressed proliferation-associated protein, Ropp 120, ROPP120DKFZp686P18118

Entrez Gene IDs

27436 (Human)

Gene Symbol

EML4

UniProt

NP_061936.2 (Human)

Additional EML4 Products

Product Documents for EML4 Antibody

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EML4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EML4 Antibody

There are currently no reviews for this product. Be the first to review EML4 Antibody and earn rewards!

Have you used EML4 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image