EMMPRIN/CD147 Antibody (JF1-045)

Novus Biologicals | Catalog # NBP2-66820

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF1-045 expressed in HEK293
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human EMMPRIN/CD147 aa 171-204 / 385. (SwissProt: P35613 Human; SwissProt: P18572 Mouse; SwissProt: P26453 Rat)

Localization

Cell membrane, Melanosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EMMPRIN/CD147 Antibody (JF1-045)

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in HeLa cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.
EMMPRIN/CD147 Antibody (JF1-045) Western Blot: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Western Blot: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820]

Western blot analysis on different lysates with NBP2-66820 at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: NCI-H226 cell lysate (15 µg/Lane) Lane 3: 786-0 cell lysate (15 µg/Lane) Lane 4: RAW264.7 cell lysate (15 µg/Lane) Lane 5: Mouse liver tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 42 kDa Observed band size: 45-60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66820 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for EMMPRIN/CD147 Antibody (JF1-045)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:5,000

Reviewed Applications

Read 1 review rated 4 using NBP2-66820 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (JF1-045)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EMMPRIN/CD147 Antibody (JF1-045)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EMMPRIN/CD147 Antibody (JF1-045) (1)

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  • EMMPRIN/CD147 Antibody (JF1-045)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human eye and Human eye cells
    Species: Human
    Verified Customer | Posted 03/22/2021
    Works good.
    Use at 1:50 (overnight at 4 degrees)
    EMMPRIN/CD147 Antibody (JF1-045) NBP2-66820

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