EMSY Antibody - BSA Free

Novus Biologicals | Catalog # NB100-545

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EMSY Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1250 and the C-terminus (residue 1322) of human EMSY chromosome 11 open reading frame 30 using the numbering given in TrEMBL entry Q8TE50 (GeneID 56946).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EMSY Antibody - BSA Free

Western Blot: EMSY Antibody [NB100-545]

Western Blot: EMSY Antibody [NB100-545]

Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545]

Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545]

Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB
Western Blot: EMSY Antibody [NB100-545]

Western Blot: EMSY Antibody [NB100-545]

Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.
Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545]

Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545]

Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: EMSY Antibody [NB100-545]

Immunoprecipitation: EMSY Antibody [NB100-545]

Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for EMSY Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:5000-1:20000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EMSY

EMSY binds BRCA2 within a region (exon 3) deleted in cancer. EMSY is capable of silencing the activation potential of BRCA2 exon 3, associates with chromatin regulators HP1 and BS69, and localizes to sites of repair following DNA damage. Its levels are amplified in breast and ovarian cancer.

Alternate Names

chromosome 11 open reading frame 30, EMSYFLJ90741, protein EMSY

Entrez Gene IDs

56946 (Human)

Gene Symbol

EMSY

UniProt

Q8TE50 (Human)

Additional EMSY Products

Product Documents for EMSY Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EMSY Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EMSY Antibody - BSA Free

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Protocols

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