Endo G Antibody - BSA Free

Western Blot: Endo G AntibodyBSA Free [NBP1-76657]

Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (A: 0.5 ug/mL, B: 0.5 ug/mL, C: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Validation of Endo G in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] -

Immunohistochemistry: Endo G Antibody - BSA Free [NBP1-76657] - Validation of Endo G in Human Pancreas Tissue. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Endo G antibody at 15 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] -

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] - Validation of Endo G in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling Endo G with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: Endo G Antibody - BSA Free [NBP1-76657] -

Knockout of ENDOG has little influence on mouse phenotype under normal conditions.a, b Body weight and liver histologic analysis in Endog+/− and Endog-/- mice under normal conditions in our lab. c Co- immunofluorescence staining of ENDOG and cytochrome c in the indicated cells (ENDOG knockout cells (KO) were transfected with wild-type ENDOG, mitochondria targeting sequence deleted ENDOG (Del 1–48), and replaced the mitochondria targeting sequence with nucleus localization sequence ENDOG (NLS-ENDOG) for 48 h; a, c, e are ENDOG knockout cells. b, d, f, are cells expressing ENDOG, Del 1–48, and NLS-ENDOG). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-51448-w), licensed under a CC-BY license. Not internally tested by Novus Biologicals.