EOMES Antibody (BLR104H) - BSA Free
Novus Biologicals | Catalog # NBP3-07014
Recombinant Monoclonal Antibody
![Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]](https://resources.rndsystems.com/images/products/EOMES-Antibody-BLR104H-Western-Blot-NBP3-07014-img0008.jpg "Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]")
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Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BLR104H
Format
BSA Free
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Product Specifications
Immunogen
Immunogen was a peptide representing a region between residue 125 and 175 of human eomesodermin homolog (EOMES) using the numbering given in entry NP_001265111.1 (Gene ID 8320).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EOMES Antibody (BLR104H) - BSA Free
Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]
Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Whole cell lysate (10 ug) from HEK293T, HeLa, NK-92, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].![Immunocytochemistry/ Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014]](https://resources.rndsystems.com/images/products/EOMES-Antibody-BLR104H-Immunocytochemistry-Immunofluorescence-NBP3-07014-img0007.jpg "Immunocytochemistry/ Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014]")
Immunocytochemistry/ Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014]
Immunocytochemistry/Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in NK-92 cells by ICC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.![Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014]](https://resources.rndsystems.com/images/products/EOMES-Antibody-BLR104H-Immunohistochemistry-NBP3-07014-img0006.jpg "Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014]")
Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014]
Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in tonsil by IHC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRP-conjugatedgoat anti-rabbit IgG. Substrate: DAB.![Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]](https://resources.rndsystems.com/images/products/EOMES-Antibody-BLR104H-Western-Blot-NBP3-07014-img0005.jpg "Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]")
Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]
Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Detection of human EOMES by WB of immunoprecipitates from NK-92 cell lysate. Antibodies: NBP3-07014 and rabbit anti-EOMES BL24367.Applications for EOMES Antibody (BLR104H) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Immunoprecipitation
6 ul/1 mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Borate Buffered Saline, pH 8.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: EOMES
Long Name
Eomesodermin Homolog
Alternate Names
Eomesodermin, TBR2
Gene Symbol
EOMES
Additional EOMES Products
Product Documents for EOMES Antibody (BLR104H) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EOMES Antibody (BLR104H) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for EOMES Antibody (BLR104H) - BSA Free
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Q: Please supply a list of eomes antibodies
A:
Here is a link to all of our EOMES antibodies
