EphB3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84776

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EphB3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVEFVP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit EphB3 Antibody - BSA Free (NBP1-84776) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EphB3 Antibody - BSA Free

Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776]

Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776]

Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining in human skin and skeletal muscle tissues using anti-EPHB3 antibody. Corresponding EPHB3 RNA-seq data are presented for the same tissues.
EphB3 Antibody

Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] -

Immunohistochemistry-Paraffin: EphB3 Antibody [NBP1-84776] - Staining of human liver shows no positivity in hepatocytes as expected.
EphB3 Antibody - BSA Free Western Blot: EphB3 Antibody - BSA Free [NBP1-84776]

Western Blot: EphB3 Antibody - BSA Free [NBP1-84776]

Analysis in control (vector only transfected HEK293T lysate) and EPHB3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401410).
EphB3 Antibody - BSA Free Immunohistochemistry-Paraffin: EphB3 Antibody - BSA Free [NBP1-84776]

Immunohistochemistry-Paraffin: EphB3 Antibody - BSA Free [NBP1-84776]

Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
EphB3 Antibody - BSA Free Immunohistochemistry-Paraffin: EphB3 Antibody - BSA Free [NBP1-84776]

Immunohistochemistry-Paraffin: EphB3 Antibody - BSA Free [NBP1-84776]

Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
EphB3 Antibody - BSA Free Immunohistochemistry-Paraffin: EphB3 Antibody - BSA Free [NBP1-84776]

Immunohistochemistry-Paraffin: EphB3 Antibody - BSA Free [NBP1-84776]

Staining of human skeletal muscle shows no positivity in myocytes.

Applications for EphB3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB3

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Long Name

Eph Receptor B3

Alternate Names

Cek10, Hek2, Mdk5, Sek4, Tyro6

Gene Symbol

EPHB3

Additional EphB3 Products

Product Documents for EphB3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EphB3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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