epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Novus Biologicals | Catalog # NBP2-41371

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Sandwich ELISA, Sandwich ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3c7

Format

BSA Free
View all epithelial Sodium Channel gamma Primary Antibodies »

Product Specifications

Immunogen

The inhibitory peptide from the human gamma-ENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human gamma-ENaC subunit.

Epitope

The epitope is on the inhibitory tract of human ENaC.

Specificity

Antibody is specific for the inhibitory tract of human gamma-ENaC subunit.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]

Western Blot: epithelial Sodium Channel gamma Antibody (3c7)BSA Free [NBP2-41371]

Western Blot: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - Analysis on a lysate of HEK293 cells transfected with and without yENaC. Lane 1 loaded with 5ug Lane 2 loaded with 10ug Lane 3 loaded with 20ug
ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]

ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371]

ELISA: epithelial Sodium Channel gamma Antibody (3c7) - BSA Free [NBP2-41371] - The calibration curve of a sandwich assay for Anti-gENaC using NBP2-41371 as the capture antibody and NBP2-41373B as the biotinylated detection antibody.

Applications for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA Capture

1-4 ug/ml

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-41371 was used in ELISA. A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131). WB: NBP2-41371 was used in Western blot. (1,2) IHC: NBP2-41371 was used in immunohistochemistry.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: epithelial Sodium Channel gamma

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq]

Long Name

Amiloride-sensitive sodium channel subunit gamma

Alternate Names

ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G

Gene Symbol

SCNN1G

Additional epithelial Sodium Channel gamma Products

Product Documents for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

Certificate of Analysis

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Product Specific Notices for epithelial Sodium Channel gamma Antibody (3c7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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