ERO1L Antibody (4G3) - Azide and BSA Free

Novus Biologicals | Catalog # H00030001-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4G3

Format

Azide and BSA Free
View all ERO1L alpha Primary Antibodies »

Product Specifications

Immunogen

ERO1L (NP_055399, 90 a.a. ~ 178 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEY

Specificity

ERO1L - ERO1-like (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ERO1L Antibody (4G3) - Azide and BSA Free

Western Blot: ERO1L Antibody (4G3) [H00030001-M01]

Western Blot: ERO1L Antibody (4G3) [H00030001-M01]

Western Blot: ERO1L Antibody (4G3) [H00030001-M01] - ERO1L monoclonal antibody (M01), clone 4G3. Analysis of ERO1L expression in PC-12.
Immunohistochemistry-Paraffin: ERO1L Antibody (4G3) [H00030001-M01]

Immunohistochemistry-Paraffin: ERO1L Antibody (4G3) [H00030001-M01]

Immunohistochemistry-Paraffin: ERO1L Antibody (4G3) [H00030001-M01] - Analysis of monoclonal antibody to ERO1L on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml.
Western Blot: ERO1L Antibody (4G3) [H00030001-M01]

Western Blot: ERO1L Antibody (4G3) [H00030001-M01]

Western Blot: ERO1L Antibody (4G3) [H00030001-M01] - ERO1L monoclonal antibody (M01), clone 4G3 Analysis of ERO1L expression in HeLa.
Western Blot: ERO1L Antibody (4G3) [H00030001-M01]

Western Blot: ERO1L Antibody (4G3) [H00030001-M01]

Western Blot: ERO1L Antibody (4G3) [H00030001-M01] - Analysis of ERO1L expression in transfected 293T cell line by ERO1L monoclonal antibody (M01), clone 4G3.Lane 1: ERO1L transfected lysate(54 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: ERO1L Antibody (4G3) [H00030001-M01] - Detection limit for recombinant GST tagged ERO1L is approximately 3ng/ml as a capture antibody.

Applications for ERO1L Antibody (4G3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERO1L alpha

Perhaps the most distinctive feature of protein folding in the ER is the abundance of disulfide bonds that must form during maturation of proteins traveling along the secretory pathway. Formation of disulfide bonds is a redox reaction. Thus, to match the flux of disulfide bonds that exit from the ER by virtue of protein secretion, a flux of oxidizing equivalents into the ER is required. In eukaryotic cells, the essential protein relay supporting this flux, and hence disulfide bond formation, involves endoplasmic reticulum oxidoreductin 1 (Ero1) and protein disulfide isomerase (PDI). The temporal pattern of hypoxic ERO1-L alpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1 alpha). We speculate from these findings that the expression of ERO1-L alpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes.

Long Name

Endoplasmic Reticulum Oxidoreductin 1 alpha

Alternate Names

ERO1-alpha, ERO1-L, ERO1-l alpha, ERO1-like, ERO1-like alpha, ERO1A, ERO1L

Entrez Gene IDs

30001 (Human)

Gene Symbol

ERO1A

UniProt

NP_055399 (Human)

Additional ERO1L alpha Products

Product Documents for ERO1L Antibody (4G3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ERO1L Antibody (4G3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for ERO1L Antibody (4G3) - Azide and BSA Free

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Protocols

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