espin Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-90588
![espin Antibody - BSA Free Immunohistochemistry: espin Antibody - BSA Free [NBP1-90588]](https://resources.rndsystems.com/images/products/nbp1-90588_rabbit-polyclonal-espin-antibody-29520251647824.jpg "Immunohistochemistry: espin Antibody - BSA Free [NBP1-90588]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Cited:
Immunohistochemistry-Paraffin, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SMPAWRRDLLRKKLEEEREQKRKEEERQKQEELRREKEQSEKLRTLGYDESKLAPWQRQVILKK
Reactivity Notes
Rat reactivity reported in (PMID:30467855). Mouse (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for espin Antibody - BSA Free
![Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]](https://resources.rndsystems.com/images/products/espin-Antibody-Immunohistochemistry-Paraffin-NBP1-90588-img0019.jpg "Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]")
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]](https://resources.rndsystems.com/images/products/espin-Antibody-Immunohistochemistry-Paraffin-NBP1-90588-img0018.jpg "Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]")
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human skeletal muscle shows no positivity in myocytes as expected.![Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]](https://resources.rndsystems.com/images/products/espin-Antibody-Immunohistochemistry-Paraffin-NBP1-90588-img0016.jpg "Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]")
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.![Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]](https://resources.rndsystems.com/images/products/espin-Antibody-Immunohistochemistry-Paraffin-NBP1-90588-img0017.jpg "Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]")
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human kidney shows strong positivity in luminal membrane in cells in tubules.![Simple Western: espin Antibody [NBP1-90588]](https://resources.rndsystems.com/images/products/espin-Antibody-Simple-Western-NBP1-90588-img0008.jpg "Simple Western: espin Antibody [NBP1-90588]")
Simple Western: espin Antibody [NBP1-90588]
Simple Western: espin Antibody [NBP1-90588] - Simple Western lane view shows a specific band for ESPN in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: espin Antibody [NBP1-90588]](https://resources.rndsystems.com/images/products/espin-Antibody-Simple-Western-NBP1-90588-img0009.jpg "Simple Western: espin Antibody [NBP1-90588]")
Simple Western: espin Antibody [NBP1-90588]
Simple Western: espin Antibody [NBP1-90588] - Electropherogram image(s) of corresponding Simple Western lane view. espin antibody was used at 1:20 dilution on RT-4 lysate(s).![espin Antibody - BSA Free Western Blot: espin Antibody - BSA Free [NBP1-90588]](https://resources.rndsystems.com/images/products/nbp1-90588_rabbit-polyclonal-espin-antibody-305202510132911.jpg "Western Blot: espin Antibody - BSA Free [NBP1-90588]")
Western Blot: espin Antibody - BSA Free [NBP1-90588]
Analysis in human cell line SK-BR-3.Applications for espin Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Simple Western
1:20
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 146 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 146 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: espin
Alternate Names
autosomal recessive deafness type 36 protein, DKFZp434A196, DKFZp434G2126, espin
Entrez Gene IDs
83715 (Human)
Gene Symbol
ESPN
UniProt
Additional espin Products
Product Documents for espin Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for espin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for espin Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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