F-box protein 43 Antibody

Novus Biologicals | Catalog # NB100-2455

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all F-box protein 43 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PGSAQSKRNLKRL corresponding to C-Terminus according to NP_001025031.2.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 19802389).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for F-box protein 43 Antibody

Western Blot: F-box protein 43 Antibody [NB100-2455]

Western Blot: F-box protein 43 Antibody [NB100-2455]

Western Blot: F-box protein 43 Antibody [NB100-2455] - Antibody at 0.3ug/ml. Mouse ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for F-box protein 43 Antibody

Application
Recommended Usage

Flow Cytometry

5 - 10 ug/ml

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Western Blot

0.3 - 1 ug/ml
Application Notes
Flow cytometry successful usage on rat meiotic testis cells is reported in scientific literature (PMID: 19802389). WB successful usage on rat pachytene spermatocytes showing a 71 kDa band is reported in scientific literature (PMID: 19802389) and in mouse oocytes is reported in scientific literature (PMID: 22302686). This antibody has been successfully used in WB in human (PMID:27605379).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: F-box protein 43

Alternate Names

early mitotic inhibitor 2, EMI2Fbx43, Endogenous meiotic inhibitor 2, ERP1, F-box only protein 43, F-box protein 43, FBX43

Entrez Gene IDs

286151 (Human); 78803 (Mouse); 315034 (Rat)

Gene Symbol

FBXO43

UniProt

NP_001025031.2. (Human)

Additional F-box protein 43 Products

Product Documents for F-box protein 43 Antibody

Certificate of Analysis

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Product Specific Notices for F-box protein 43 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for F-box protein 43 Antibody

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Protocols

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