FBXL2 Antibody

Novus Biologicals | Catalog # NB100-1289

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence VFSNNDEGLINKK-C corresponding to N-Terminus according to NP_036289.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FBXL2 Antibody

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NB100-1289]

Immunocytochemistry/ Immunofluorescence: FBXL2 Antibody [NB100-1289]

Immunocytochemistry/Immunofluorescence: FBXL2 Antibody [NB100-1289] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289]

Immunohistochemistry-Paraffin: FBXL2 Antibody [NB100-1289] - Staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Antibody at 8 ug/mL.

Applications for FBXL2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

8 ug/mL

Immunohistochemistry-Paraffin

8 ug/mL

Peptide ELISA

Detection limit 1:128000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FBXL2

FBXL2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.

Alternate Names

FBL2FBL3F-box protein containing leucine-rich repeats, F-box and leucine-rich repeat protein 2DKFZp564P0622, F-box protein FBL2/FBL3, F-box/LRR-repeat protein 2

Entrez Gene IDs

25827 (Human)

Gene Symbol

FBXL2

Additional FBXL2 Products

Product Documents for FBXL2 Antibody

Certificate of Analysis

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Product Specific Notices for FBXL2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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