Frequenin Antibody (24GB25)

Novus Biologicals | Catalog # NBP3-51412

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB25
View all Frequenin Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Frequenin (Uniprot: P62166)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Frequenin Antibody (24GB25)

Frequenin Antibody (24GB25)

Immunocytochemistry/ Immunofluorescence: Frequenin Antibody (24GB25)[NBP3-51412] -

Frequenin antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: neuronal calcium sensor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Frequenin Antibody (24GB25)

Immunocytochemistry/ Immunofluorescence: Frequenin Antibody (24GB25)[NBP3-51412] -

Immunocytochemical staining of HepG2 cells with Frequenin antibody (1:1,000). Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Frequenin Antibody (24GB25)

Flow Cytometry: Frequenin Antibody (24GB25)[NBP3-51412] -

Validation of Frequenin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Frequenin antibody (1:2,000) and analyzed using BD flow cytometer.
Frequenin Antibody (24GB25)

Flow Cytometry: Frequenin Antibody (24GB25)[NBP3-51412] -

Flow cytometric analysis of Frequeninexpression in HepG2 cells using Frequenin antibody (1:2,000). Green, isotype control; red, Neuronal calcium sensor 1.
Frequenin Antibody (24GB25)

Western Blot: Frequenin Antibody (24GB25)[NBP3-51412] -

Western blotting analysis using Frequenin antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for Frequenin Antibody (24GB25)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Frequenin

Frequenin is a Ca2+ binding protein implicated in the regulation of neurotransmitter release at the neuromuscular junction. Frequenin amino acid sequences from different species appear closely related. In mouse a single 4.2 kbp mRNA is produced yielding a protein of 22 kDa when assayed by SDS-PAGE under reducing conditions. Frequenin may colocalize with the dendritic marker MAP-2 and is widely distributed in the brain, spinal cord and dorsal root ganglia. In Drosophila frequenin is related to recoverin and visinin and functions like Ca2+-sensitive guanylyl cyclase activator.

Alternate Names

DKFZp761L1223, FREQFLUP, frequenin (Drosophila) homolog, Frequenin homolog, frequenin homolog (Drosophila), Frequenin-like protein, Frequenin-like ubiquitous protein, NCS-1, neuronal calcium sensor 1

Gene Symbol

NCS1

Additional Frequenin Products

Product Documents for Frequenin Antibody (24GB25)

Certificate of Analysis

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Product Specific Notices for Frequenin Antibody (24GB25)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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