FRMD5 Antibody (CL9503) - BSA Free
Novus Biologicals | Catalog # NBP2-88935
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (98%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL9503
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q7Z6J6, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for FRMD5 Antibody (CL9503) - BSA Free
![Western Blot: FRMD5 Antibody (CL9503) [NBP2-88935]](https://resources.rndsystems.com/images/products/FRMD5-Antibody-CL9503-Western-Blot-NBP2-88935-img0006.jpg "Western Blot: FRMD5 Antibody (CL9503) [NBP2-88935]")
Western Blot: FRMD5 Antibody (CL9503) [NBP2-88935]
Western Blot: FRMD5 Antibody (CL9503) [NBP2-88935] - Analysis in human cell line SCLC-21H.![Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]](https://resources.rndsystems.com/images/products/FRMD5-Antibody-CL9503-Immunohistochemistry-Paraffin-NBP2-88935-img0005.jpg "Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]")
![Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]](https://resources.rndsystems.com/images/products/FRMD5-Antibody-CL9503-Immunohistochemistry-Paraffin-NBP2-88935-img0001.jpg "Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]")
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935] - Staining of human cerebral cortex shows weak to moderate positivity in neuronal processes.![Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]](https://resources.rndsystems.com/images/products/FRMD5-Antibody-CL9503-Immunohistochemistry-Paraffin-NBP2-88935-img0002.jpg "Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]")
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935] - Staining of human colon shows no positivity in glandular cells as expected.![Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]](https://resources.rndsystems.com/images/products/FRMD5-Antibody-CL9503-Immunohistochemistry-Paraffin-NBP2-88935-img0003.jpg "Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]")
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.![Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]](https://resources.rndsystems.com/images/products/FRMD5-Antibody-CL9503-Immunohistochemistry-Paraffin-NBP2-88935-img0004.jpg "Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]")
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935]
Immunohistochemistry-Paraffin: FRMD5 Antibody (CL9503) [NBP2-88935] - Staining of human placenta shows no positivity in trophoblastic cells as expected.Applications for FRMD5 Antibody (CL9503) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FRMD5
Alternate Names
FERM domain containing 5, FERM domain-containing protein 5, FLJ41022, MGC14161
Gene Symbol
FRMD5
Additional FRMD5 Products
Product Documents for FRMD5 Antibody (CL9503) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FRMD5 Antibody (CL9503) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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