FTO Antibody (FT62-6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80050

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FT62-6

Format

Azide and BSA Free
View all FTO Primary Antibodies »

Product Specifications

Immunogen

Recombinant human FTO.

Specificity

Recognizes mouse FTO. Weakly cross-reacts with human FTO. Does not cross-react with rat FTO.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FTO Antibody (FT62-6) - Azide and BSA Free

Western Blot: FTO Antibody (FT62-6)Azide and BSA Free [NBP2-80050]

Western Blot: FTO Antibody (FT62-6)Azide and BSA Free [NBP2-80050]

Western Blot: FTO Antibody (FT62-6) [NBP2-80050] - Analysis using anti-FTO (mouse), mAb (FT62-6) at 1:2'000 dilution.A) 1: Mouse kideny (200 ug). 2: Mouse spleen (200 ug). 3: Mouse heart (200 ug). 4: Mouse liver (500 ug). 5: Mouse stomach (200 ug). 6: Mouse brain (300 ug). 7: Mouse lung (200 ug). B) 1: Mouse FTO (10 ng). 2: 3T3L1 (100 ug).
Immunohistochemistry-Paraffin: FTO Antibody (FT62-6) - Azide and BSA Free [NBP2-80050]

Immunohistochemistry-Paraffin: FTO Antibody (FT62-6) - Azide and BSA Free [NBP2-80050]

Immunohistochemistry-Paraffin: FTO Antibody (FT62-6) [NBP2-80050] - Immunohistochemical staining of mouse FTO using anti-FTO (mouse), mAb (FT62-6) in mouse tissue (1:500 dilution, 200X). A. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse brain. A-1. Isotype control, mouse brain. B. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse uterus. B-1. Isotype control, mouse uterus.
Immunoprecipitation: FTO Antibody (FT62-6) - Azide and BSA Free [NBP2-80050]

Immunoprecipitation: FTO Antibody (FT62-6) - Azide and BSA Free [NBP2-80050]

Immunoprecipitation: FTO Antibody (FT62-6) [NBP2-80050] - Immunoprecipitation confirmed by recombinant mouse FTO. Recombinant mouse FTO proteins at different concentrations were precipitated using anti-FTO (mouse), mAb (FT62-6). The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb. 1. mFTO (His-tagged) recombinant control 50 ng. 2. mFTO (His-tagged) 10 ug. 3. mFTO (His-tagged) 5 ug. 4. mFTO (His-tagged) 2.5 ug. 5. mFTO (His-tagged) 1.25 ug. 6. mFTO (His-tagged) 0.312 ug. 7. mFTO (His-tagged) 0.156 ug.

Applications for FTO Antibody (FT62-6) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:2000 - 1:5000

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

5 ug/mL

Western Blot

1:2000 - 1:5000
Application Notes
Immunoprecipitation: 5ug/ml per sample in a final reaction volume of 500-1000 ul.

Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um-filtered solution in PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FTO

Fat mass and obesity-associated protein (FTO) is a novel protein and a member of the non-heme dioxygenase (Fe(II)- and 2-oxoglutarate-dependent dioxygenases) superfamily. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.

FTO antibodies are useful tools for lipid metabolism research and obesity studies.

Long Name

Fat Mass and Obesity Associated

Alternate Names

Alpha-ketoglutarate-dependent dioxygenase FTO

Gene Symbol

FTO

Additional FTO Products

Product Documents for FTO Antibody (FT62-6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FTO Antibody (FT62-6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes
Obesity

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FTO Antibody (FT62-6) - Azide and BSA Free

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  • Q: Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?

    A: With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.

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