GEF-H1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-21577

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%), Canine (92%), Porcine (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GEF-H1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.

Localization

Cytoplasm; Cell junction; tight junction; Golgi apparatus; Spindle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GEF-H1 Antibody - BSA Free

Western Blot: GEF-H1 Antibody [NBP2-21577]

Western Blot: GEF-H1 Antibody [NBP2-21577]

Western Blot: GEF-H1 Antibody [NBP2-21577] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: GEF-H1 Antibody [NBP2-21577]

Immunocytochemistry/ Immunofluorescence: GEF-H1 Antibody [NBP2-21577]

Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody [NBP2-21577] - GEF-H1 antibody detects GEF-H1 protein at cytoskeleton and Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577]

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577]

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - GEF-H1 antibody detects GEF-H1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GEF-H1 stained by GEF-H1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: GEF-H1 Antibody [NBP2-21577]

Western Blot: GEF-H1 Antibody [NBP2-21577]

Western Blot: GEF-H1 Antibody [NBP2-21577] - Sample (10 ug of whole cell lysate) A: hMSC-3A6 B: siRNA of ARHGEF2/GEF-H1 8% SDS PAGE diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577]

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577]

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577]

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577]

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Paraffin-embedded rat hind brain. GEF-H1 antibody dilution: 1:500.
GEF-H1 Antibody - BSA Free

Immunohistochemistry-Frozen: GEF-H1 Antibody [NBP2-21577] -

GEF-H1 antibody detects GEF-H1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: GEF-H1 stained by GEF-H1 antibody (NBP2-21577) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI. br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
GEF-H1 Antibody - BSA Free

Immunohistochemistry: GEF-H1 Antibody [NBP2-21577] -

GEF-H1 antibody detects GEF-H1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GEF-H1 antibody (NBP2-21577) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
GEF-H1 Antibody - BSA Free

Western Blot: GEF-H1 Antibody [NBP2-21577] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (NBP2-21577) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GEF-H1 Antibody - BSA Free

Western Blot: GEF-H1 Antibody [NBP2-21577] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (NBP2-21577) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GEF-H1 Antibody - BSA Free

Western Blot: GEF-H1 Antibody [NBP2-21577] -

GEF-H1 antibody detects GEF-H1 protein by western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug BCL-1 whole cell lysate/extract
F. 30 ug Raw 264.7 whole cell lysate/extract
G. 30 ug C2Cl2 whole cell lysate/extract
5% SDS-PAGE
GEF-H1 antibody (NBP2-21577) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GEF-H1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GEF-H1

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Alternate Names

GEF, GEFH1, GEF-H1DKFZp547P1516, Guanine nucleotide exchange factor H1, KIAA0651DKFZp547L106, LFP40FLJ45440, Microtubule-regulated Rho-GEF, P40, Proliferating cell nucleolar antigen p40, rho guanine nucleotide exchange factor 2, Rho/Rac guanine nucleotide exchange factor (GEF) 2, Rho/Rac guanine nucleotide exchange factor 2

Gene Symbol

ARHGEF2

Additional GEF-H1 Products

Product Documents for GEF-H1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GEF-H1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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