Geminin Antibody (1A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00051053-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A8

Format

Azide and BSA Free
View all Geminin Primary Antibodies »

Product Specifications

Immunogen

GMNN (AAH05185, 110 a.a. ~ 209 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQYMAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVSSSTDAKPCI

Specificity

GMNN - geminin, DNA replication inhibitor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Geminin Antibody (1A8) - Azide and BSA Free

Western Blot: Geminin Antibody (1A8) [H00051053-M01]

Western Blot: Geminin Antibody (1A8) [H00051053-M01]

Western Blot: Geminin Antibody (1A8) [H00051053-M01] - Analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M01), clone 1A8.Lane 1: GMNN transfected lysate(23.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01]

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01]

Immunocytochemistry/Immunofluorescence: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01]

Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01]

Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1 ug/ml.
Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01]

Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01]

Immunohistochemistry-Paraffin: Geminin Antibody (1A8) [H00051053-M01] - Analysis of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
ELISA: Geminin Antibody (1A8) [H00051053-M01]

ELISA: Geminin Antibody (1A8) [H00051053-M01]

ELISA: Geminin Antibody (1A8) [H00051053-M01] - Detection limit for recombinant GST tagged GMNN is approximately 0.03ng/ml as a capture antibody.

Applications for Geminin Antibody (1A8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Geminin

Geminin is a multifunctional nuclear protein invloved in embyro development/differentiation, cell cycle regulation and DNA replciation. Originally defined as an inhibitor of DNA replication and substrate of the anaphase promoting complex, Geminin has also been shown to expand the neural plate in the developing embryos.

Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.

Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Entrez Gene IDs

51053 (Human)

Gene Symbol

GMNN

OMIM

602842 (Human)

UniProt

AAH05185 (Human)

Additional Geminin Products

Product Documents for Geminin Antibody (1A8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Geminin Antibody (1A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Geminin Antibody (1A8) - Azide and BSA Free

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Protocols

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FAQs

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