Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00051738-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F4

Format

Azide and BSA Free
View all Ghrelin/Obestatin Primary Antibodies »

Product Specifications

Immunogen

GHRL (AAH25791.1, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDEMEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Specificity

GHRL (2F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free (H00051738-M01) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-Ghrelin/Obestatin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free

Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]

Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]

Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - GHRL monoclonal antibody (M01), clone 2F4. Analysis of GHRL expression in HeLa.
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]

Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]

Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - Analysis of monoclonal antibody to GHRL on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.
ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]

ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01]

ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.

Applications for Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ghrelin/Obestatin

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]

Alternate Names

appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46

Entrez Gene IDs

51738 (Human)

Gene Symbol

GHRL

OMIM

601665 (Human)

UniProt

AAH25791 (Human)

Additional Ghrelin/Obestatin Products

Product Documents for Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Nutrition and Appetite
Obesity

Citations for Ghrelin/Obestatin Antibody (2F4) - Azide and BSA Free

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Protocols

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FAQs

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