GIT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31447

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GIT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GIT1. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GIT1 Antibody - BSA Free (NBP1-31447) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GIT1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GIT1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody [NBP1-31447]

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody [NBP1-31447]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-31447] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-31447]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-31447]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-31447] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.
Western Blot: GIT1 Antibody [NBP1-31447]

Western Blot: GIT1 Antibody [NBP1-31447]

Western Blot: GIT1 Antibody [NBP1-31447] - Rat2 whole cell lysate/extract.
GIT1 Antibody - BSA Free

Western Blot: GIT1 Antibody [NBP1-31447] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (NBP1-31447) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.
GIT1 Antibody - BSA Free

Western Blot: GIT1 Antibody [NBP1-31447] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (NBP1-31447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GIT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GIT1

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Gene Symbol

GIT1

Additional GIT1 Products

Product Documents for GIT1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GIT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for GIT1 Antibody - BSA Free

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Protocols

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