GLDC Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38682

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLDC Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SCTMKLNSSSELAPITWKEFANIHPFVPLDQAQGYQQLFRELEKDLCELTGYDQVCFQPNSGAQGEYAGLATIRAYLNQKGEGHRTVCLIPKSAHGTNPASAHMAGMKIQPVEVDKYGNIDAVHLKAMVDKHKENL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLDC Antibody - BSA Free

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682] - Analysis in human kidney and cervix, uterine tissues using NBP2-38682 antibody. Corresponding GLDC RNA-seq data are presented for the same tissues.
Western Blot: GLDC Antibody [NBP2-38682]

Western Blot: GLDC Antibody [NBP2-38682]

Western Blot: GLDC Antibody [NBP2-38682] - Analysis in human cell lines Caco-2 and HeLa using anti-GLDC antibody. Corresponding GLDC RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: GLDC Antibody [NBP2-38682]

Immunocytochemistry/ Immunofluorescence: GLDC Antibody [NBP2-38682]

Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP2-38682] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus & mitochondria.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682]

Immunohistochemistry-Paraffin: GLDC Antibody [NBP2-38682] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.

Applications for GLDC Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLDC

The enzyme system for cleavage of glycine (glycine cleavage system; GCS; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase),H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (alipoamide dehydrogenase). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes;see MIM 238310, MIM 238330, and MIM 238331.(supplied by OMIM)

Alternate Names

EC 1.4.4.2, GCE, GCSPMGC138198, Glycine cleavage system P protein, glycine cleavage system protein P, Glycine decarboxylase, glycine decarboxylase P-protein, glycine dehydrogenase (decarboxylating), glycine dehydrogenase (decarboxylating; glycine decarboxylase, glycine cleavagesystem protein P), glycine dehydrogenase [decarboxylating], mitochondrial, HYGN1, MGC138200, NKH

Gene Symbol

GLDC

UniProt

P23378 (Human)

Additional GLDC Products

Product Documents for GLDC Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLDC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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