GLI-1 Antibody (JF09-08)

Novus Biologicals | Catalog # NBP2-66867

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF09-08 expressed in HEK293
View all GLI-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human GLI-1 aa 38-82 / 1106. (SwissProt: P08151 Human; SwissProt: P47806 Mouse; Entrez Gene: 140589 Rat)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-1 Antibody (JF09-08)

Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867]

Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867]

Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867] - Western blot analysis of GLI-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: SH-SY5Y cell lysate
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867] - Analysis of paraffin-embedded rat testis tissue using anti-Gli1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867]

Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-Gli1 antibody. Counter stained with hematoxylin.
GLI-1 Antibody (JF09-08) Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867]

Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867]

Western blot analysis on different lysates with NBP2-66867 at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 118 kDa Observed band size: 150 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66867 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for GLI-1 Antibody (JF09-08)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-1

Gli1 was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that GLI is expressed in embryonal carcinoma cells but not in most adult tissue. GLI has been localized to 12q13-q14.3 by Southern blot analysis. On mouse the gene is located on chromosome 10. In mice, 3 zinc finger transcription factors, Gli-1, Gl-i2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli1 expression is central and probably sufficient for tumor development in humans.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Gene Symbol

GLI1

Additional GLI-1 Products

Product Documents for GLI-1 Antibody (JF09-08)

Certificate of Analysis

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Product Specific Notices for GLI-1 Antibody (JF09-08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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