GliS2 Antibody - BSA Free

Western Blot: GliS2 AntibodyBSA Free [NBP2-41311]

Western Blot: GliS2 Antibody [NBP2-41311] - Analysis of GLIS2 in K562 cell lysate with GLIS2 antibody at 1 ug/ml.

Immunohistochemistry: GliS2 Antibody - BSA Free [NBP2-41311] -

Immunohistochemistry: GliS2 Antibody - BSA Free [NBP2-41311] - Immunohistochemistry of GliS2 in mouse kidney tissue with GliS2 antibody at 5 ug/ml.

Knockdown Validated: GliS2 Antibody - BSA Free [NBP2-41311] -

A) (Left panel) Relative Glis2 mRNA accumulation normalized to beta ‐actin on GC1spgKO‐TGR5 cells transfected with siCtrl or siGlis2. siCtrl‐transfected cells were arbitrarily set at 1. (Right panel) Representative western blots of GLIS2 in GC1spgKO‐TGR5 cells transfected with siCtrl or siGlis2. Normalization was performed against total protein using stain‐free gels. B) Number of adherent GC1spgKO‐TGR5 cells transfected with siCtrl or siGlis2 and treated with vehicle or Bu for 24 h. Vehicle treated cells of each genotype were arbitrarily set at 1. C) Representative western blots of GLIS2 and Phospho‐TP53 (P‐TP53), and quantification of ratios in GC1spgKO‐Tgr5 cells transfected with siCtrl or siGlis2 and treated with vehicle or Bu for 24 h. Normalization was performed against total protein using stain‐free gels. Vehicle groups of each genotype were arbitrarily set at 1. D) Fgfr2, Adgrg1 and Phlda3 mRNA accumulations normalized to beta ‐actin on GC1spgKO‐TGR5 cells transfected with siCtrl or siGlis2 and treated 24h with vehicle or with 200 × 10−6 m of Bu. Vehicle groups of each genotype were arbitrarily set at 1. In all panels, n = 10–15 from 3 to 5 independent experiments. Data are expressed as the means +/- SEM. ANOVA2 followed by Holm‐Sidak's test for multiple comparisons. *, p < 0.05; **, p < 0.01 versus respective vehicle group for each genotype. #, p < 0.05 between genotypes exposed to same treatments. The horizontal square brackets underline the groups statistically compared between two conditions of different genotypes. Veh: vehicle and Bu: Busulfan. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35435331), licensed under a CC-BY license. Not internally tested by Novus Biologicals.