Glucose Oxidase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-44186

Novus Biologicals
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Key Product Details

Species Reactivity

Fungi

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
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Product Specifications

Immunogen

Glucose Oxidase [Aspergillus niger] (Uniprot: P13006)

Reactivity Notes

Reactive with Glucose Oxidase from Aspergillus niger.
Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This Anti-Glucose Oxidase Antibody product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glucose Oxidase Antibody - BSA Free

Western Blot: Glucose Oxidase Antibody [NBP2-44186]

Western Blot: Glucose Oxidase Antibody [NBP2-44186]

Western Blot: Glucose Oxidase Antibody [NBP2-44186] - Analysis under reducing and non-reducing conditions.
Western Blot: Glucose Oxidase Antibody [NBP2-44186]

Western Blot: Glucose Oxidase Antibody [NBP2-44186]

Western Blot: Glucose Oxidase Antibody [NBP2-44186] - Analysis under reducing and non-reducing conditions. Reduced samples of purified Glucose Oxidase contained 4% BME and were boiled for 5 minutes. Samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti Glucose Oxidase antibody.

Applications for Glucose Oxidase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:10-1:500

Western Blot

1:1000-1:5000
Application Notes
This product has been tested in western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucose Oxidase

Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.

Alternate Names

Beta D Glucose Oxygen 1 Oxido Reductase, Glucose Oxyhydrase, GOD

UniProt

Additional Glucose Oxidase Products

Product Documents for Glucose Oxidase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glucose Oxidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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