Key Product Details

Species Reactivity

Fungi

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein. (Uniprot: P13006)

Reactivity Notes

Reactive with Glucose Oxidase from Aspergillus niger.
Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glucose Oxidase Antibody - BSA Free

Western Blot: Glucose Oxidase Antibody [NBP2-44232]

Western Blot: Glucose Oxidase Antibody [NBP2-44232]

Western Blot: Glucose Oxidase Antibody [NBP2-44232] - lane 1: Glucose Oxidase. Lane 2: None. Load: 50 ng per lane.

Applications for Glucose Oxidase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:35000-1:160000

Immunohistochemistry

1:2000-1:8000

Western Blot

1:3500-1:16000
Application Notes
This product has been tested by ELISA and western blot and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucose Oxidase

Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.

Alternate Names

Beta D Glucose Oxygen 1 Oxido Reductase, Glucose Oxyhydrase, GOD

UniProt

Additional Glucose Oxidase Products

Product Documents for Glucose Oxidase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Glucose Oxidase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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