Glutathione Synthetase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57177

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glutathione Synthetase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPG

Reactivity Notes

Mouse (82%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutathione Synthetase Antibody - BSA Free

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex, colon, epididymis and testis using Anti-GSS antibody NBP2-57177 (A) shows similar protein distribution across tissues to independent antibody NBP2-30465 (B).
Western Blot: Glutathione Synthetase Antibody [NBP2-57177]

Western Blot: Glutathione Synthetase Antibody [NBP2-57177]

Western Blot: Glutathione Synthetase Antibody [NBP2-57177] - Analysis using Anti-GSS antibody NBP2-57177 (A) shows similar pattern to independent antibody NBP2-30465 (B).
Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177]

Immunocytochemistry/ Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177]

Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human testis.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human colon.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177]

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human epididymis using Anti-GSS antibody NBP2-57177.

Applications for Glutathione Synthetase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. See Simple Western Antibody Database for Simple Western validation: separated by Size

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Synthetase

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage byfree radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as ahomodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion ofgamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.(provided by RefSeq)

Alternate Names

EC 6.3.2.3, Glutathione synthase, glutathione synthetase, GSH synthetase, GSHS, GSH-S, MGC14098

Gene Symbol

GSS

Additional Glutathione Synthetase Products

Product Documents for Glutathione Synthetase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glutathione Synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutathione Synthetase Antibody - BSA Free

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Protocols

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FAQs

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