Granulin Antibody - BSA Free

Western Blot: Granulin Antibody - BSA Free [NBP1-32076] -

Deletion of progranulin suppressed apoptosis in the airways exposed to ovalbumin, but aggravated the human bronchial epithelial cells apoptosis in vitro. (A) Representative images of lung sections from WT and PGRN KO mice stained for TUNEL. Scale bar: 100 μm. (B) The protein levels of cleaved caspase 3 in mice lung were analyzed by Western blot. The results of densitometric analysis were showed as means +/- SD (n = 3). *p <.05, unpaired t test. (C) Western blot analysis of PGRN and cleaved caspase 3 in 16‐HBE cells treated (or not) with H2O2 for 48 h. The results of densitometric analysis were showed as means +/- SD (n = 3). ****p <.0001, one‐way ANOVA. (D) The knockdown efficiency of siRNA targeting PGRN was identified at the mRNA and protein levels 24 and 48 h following transfection, respectively. The relative mRNA expression and densitometric analysis were showed as means +/- SD (n = 3). ****p <.0001, one‐way ANOVA. (E) Following knockdown of PGRN for 24 h, 16‐HBE cells were treated (or not) with H2O2 for 24 h. The densitometric analysis of cleaved caspase 3 was presented as means +/- SD (n = 3). *p <.05, unpaired t test. The study was repeated for three times. ANOVA, analysis of variance; DAPI, 4′,6‐diamidino‐2‐phenylindole; HBE, human bronchial epithelial cells; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36840485), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Granulin Antibody - BSA Free [NBP1-32076] -

Deletion of progranulin suppressed apoptosis in the airways exposed to ovalbumin, but aggravated the human bronchial epithelial cells apoptosis in vitro. (A) Representative images of lung sections from WT and PGRN KO mice stained for TUNEL. Scale bar: 100 μm. (B) The protein levels of cleaved caspase 3 in mice lung were analyzed by Western blot. The results of densitometric analysis were showed as means +/- SD (n = 3). *p <.05, unpaired t test. (C) Western blot analysis of PGRN and cleaved caspase 3 in 16‐HBE cells treated (or not) with H2O2 for 48 h. The results of densitometric analysis were showed as means +/- SD (n = 3). ****p <.0001, one‐way ANOVA. (D) The knockdown efficiency of siRNA targeting PGRN was identified at the mRNA and protein levels 24 and 48 h following transfection, respectively. The relative mRNA expression and densitometric analysis were showed as means +/- SD (n = 3). ****p <.0001, one‐way ANOVA. (E) Following knockdown of PGRN for 24 h, 16‐HBE cells were treated (or not) with H2O2 for 24 h. The densitometric analysis of cleaved caspase 3 was presented as means +/- SD (n = 3). *p <.05, unpaired t test. The study was repeated for three times. ANOVA, analysis of variance; DAPI, 4′,6‐diamidino‐2‐phenylindole; HBE, human bronchial epithelial cells; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36840485), licensed under a CC-BY license. Not internally tested by Novus Biologicals.