Granzyme H Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86565

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Granzyme H Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HPAYNPKNFSNDIMLLQLERKAKWTTAVRPLRLPSSKAQVKPGQLCSVAGWGYVSMSTLATTLQEVLLTVQKDCQCERLFHGNYSRATEICVGDPKKTQTGFKGDSGGPLVCKDVAQGILSYGNKKGTPPGVYIKVSHFLPWIKRTM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Granzyme H Antibody - BSA Free (NBP1-86565) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Granzyme H Antibody - BSA Free

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody [NBP1-86565] - Staining in human spleen and pancreas tissues using anti-GZMH antibody. Corresponding GZMH RNA-seq data are presented for the same tissues.
Granzyme H Antibody - BSA Free Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Granzyme H Antibody - BSA Free Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Staining of human tonsil shows strong granular cytoplasmic positivity in non-germinal center cells.
Granzyme H Antibody - BSA Free Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Granzyme H Antibody - BSA Free Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Immunohistochemistry-Paraffin: Granzyme H Antibody - BSA Free [NBP1-86565]

Staining of human spleen shows moderate granular positivity in cells in red pulp.
Granzyme H Antibody - BSA Free Western Blot: Granzyme H Antibody - BSA Free [NBP1-86565]

Western Blot: Granzyme H Antibody - BSA Free [NBP1-86565]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)

Applications for Granzyme H Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Granzyme H

The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses.

Alternate Names

CCP-X, CGL2, CTSGL2, GrzH, GZMH

Entrez Gene IDs

2999 (Human)

Gene Symbol

GZMH

UniProt

P20718

Additional Granzyme H Products

Product Documents for Granzyme H Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Granzyme H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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