Guinea Pig TNF-alpha Antibody (569806) [Biotin]

Name: Guinea Pig TNF-alpha Antibody (569806) [Biotin]
Brand: R&D Systems
SKU: BAM50351
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # BAM50351

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Guinea Pig

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 569806

View all TNF-alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant guinea pig TNF-alpha
Leu79-Leu234
Accession # P51435

Specificity

Detects recombinant guinea pig TNF-alpha in ELISAs. In sandwich immunoassays, no cross-reactivity with human, mouse, rat, porcine, canine, feline, equine, bovine, or cotton rat TNF-alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Guinea Pig TNF-alpha Antibody (569806) [Biotin]

Guinea Pig TNF-alpha ELISA Standard Curve

Recombinant Guinea Pig TNF‑ alpha (Catalog # 5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Guinea Pig TNF-alpha Antibody (569806) [Biotin]

Guinea Pig TNF-alpha Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Capture Reagent: Guinea Pig TNF‑ alpha Antibody (Catalog # MAB5035)
Standard: Recombinant Guinea Pig TNF-alpha Protein (Catalog # 5035-TG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Guinea pig TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 155 aa extracellular domain (ECD) (3). Within the ECD, guinea pig TNF-alpha shares 80% - 83% aa sequence identity with human, mouse, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

References

Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.
Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
White, A.M. et al. (1997) Am. J. Physiol. 273:L524.
Tang, P. et al. (1996) Biochemistry 35:8216.
Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
Black, R.A. et al. (1997) Nature 385:729.
Moss, M.L. et al. (1997) Nature 385:733.
Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
Romanatto, T. et al. (2007) Peptides 28:1050.
Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)