H4 Clustered Histone 1 [ac Lys5] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35514

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Chromatin Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all H4 Clustered Histone 1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human H4 Clustered Histone 1 (NP_001029249.1).

Sequence:
GRGKG

Modification

ac Lys 5

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for H4 Clustered Histone 1 [ac Lys5] Antibody - BSA Free

H4 Clustered Histone 1 [ac Lys5] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
H4 Clustered Histone 1 [ac Lys5] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] - Immunohistochemistry analysis of paraffin-embedded Human lung using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
H4 Clustered Histone 1 [ac Lys5] Antibody

Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] -

Chromatin Immunoprecipitation: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using H4 Clustered Histone 1 Rabbit pAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
H4 Clustered Histone 1 [ac Lys5] Antibody

Western Blot: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] -

Western Blot: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
H4 Clustered Histone 1 [ac Lys5] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys5] Antibody [NBP3-35514] - Immunohistochemistry analysis of paraffin-embedded Rat lung using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Applications for H4 Clustered Histone 1 [ac Lys5] Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

5ug antibody for 5ug-10ug of Chromatin

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: H4 Clustered Histone 1

Alternate Names

H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA, H4F4, HIST1H4A

Gene Symbol

H4C1

Additional H4 Clustered Histone 1 Products

Product Documents for H4 Clustered Histone 1 [ac Lys5] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for H4 Clustered Histone 1 [ac Lys5] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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