HA Tag Biotinylated Antibody

Recombinant Monoclonal Antibody
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HA Tag Biotinylated Antibody in Data
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Product Details
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HA Tag Biotinylated Antibody Summary

Detects N-terminal and C-terminal HA Tag proteins in Western blot.
Recombinant Monoclonal Rabbit IgG Clone # 1049F
Protein A or G purified from cell culture supernatant
HA Tag Peptide
Accession # ABB51961
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.


Recommended Concentration
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of HA-tagged Proteins by Western Blot. View Larger

Detection of HA-tagged Proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Tag Biotinylated Monoclonal Antibody (Catalog # BAM0601) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for HA-tagged proteins at approximately 70 and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HA Tag

Entrez Gene IDs
ABB51961.1 (Viral)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets


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