HA Tag Antibody (1049F) [Biotin]

R&D Systems | Catalog # BAM0601

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1049F
View all HA Tag Primary Antibodies »

Product Specifications

Immunogen

HA Tag Peptide
Accession # ABB51961

Specificity

Detects N-terminal and C-terminal HA Tag proteins in Western blot.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HA Tag Antibody (1049F) [Biotin]

Detection of HA-tagged Proteins antibody by Western Blot.

Detection of HA-tagged Proteins by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Tag Biotinylated Monoclonal Antibody (Catalog # BAM0601) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for HA-tagged proteins at approximately 70 and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for HA Tag Antibody (1049F) [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Entrez Gene IDs

23308115 (Influenza A Virus H1N1); 3655151 (Influenza A Virus H3N2)

Gene Symbol

HA

UniProt

ABB51961

Additional HA Tag Products

Product Documents for HA Tag Antibody (1049F) [Biotin]

Certificate of Analysis

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Product Specific Notices for HA Tag Antibody (1049F) [Biotin]

For research use only

Related Research Areas

Infectious Diseases

Citations for HA Tag Antibody (1049F) [Biotin]

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