HDJ2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88019

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDJ2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDJ2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HDJ2 Antibody [NBP1-88019]

Immunocytochemistry/ Immunofluorescence: HDJ2 Antibody [NBP1-88019]

Immunocytochemistry/Immunofluorescence: HDJ2 Antibody [NBP1-88019] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019]

Immunohistochemistry-Paraffin: HDJ2 Antibody [NBP1-88019] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.
HDJ2 Antibody - BSA Free Western Blot: HDJ2 Antibody - BSA Free [NBP1-88019]

Western Blot: HDJ2 Antibody - BSA Free [NBP1-88019]

Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-DNAJA1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
HDJ2 Antibody - BSA Free Western Blot: HDJ2 Antibody - BSA Free [NBP1-88019]

Western Blot: HDJ2 Antibody - BSA Free [NBP1-88019]

Analysis in mouse cell line NIH-3T3.

Applications for HDJ2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDJ2

HDJ 2 is a novel human homolog of E. coli's DNA J protein from heat shock protein 40 family. Hsp 40 is involved in chaperonic protein folding. HDJ-2 is cytoplasmic and nuclear membrane associated protein; upon heat shock it migrates and becomes associated with the golgi complex, nucleous and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.

Alternate Names

DJ-2, DjA1, DnaJ (Hsp40) homolog, subfamily A, member 1, dnaJ homolog subfamily A member 1, DnaJ protein homolog 2, DNAJ2, HDJ2, hDj-2, Heat shock 40 kDa protein 4, Heat shock protein J2, heat shock protein, DNAJ-like 2, HSDJ, HSJ2, HSJ-2, HSPF4hdj-2, Human DnaJ protein 2, NEDD7, neural precursor cell expressed, developmentally down-regulated 7

Gene Symbol

DNAJA1

Additional HDJ2 Products

Product Documents for HDJ2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HDJ2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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