Helicobacter Pylori Antibody (MSVA-466M)

Novus Biologicals | Catalog # NBP3-57263

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MSVA-466M
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Product Specifications

Immunogen

Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary) Swissprot Not Applicable

Localization

Cell surface. Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands..

Negative Control
Negitve control:Gastric tissue: Normal epithelial and stromal cells should not stain.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Applications for Helicobacter Pylori Antibody (MSVA-466M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Helicobacter Pylori

This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Alternate Names

H. pylori

Additional Helicobacter Pylori Products

Product Documents for Helicobacter Pylori Antibody (MSVA-466M)

Certificate of Analysis

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Product Specific Notices for Helicobacter Pylori Antibody (MSVA-466M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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