Helicobacter Pylori Antibody (MSVA-466M) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57348

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MSVA-466M

Format

Azide and BSA Free
View all Helicobacter Pylori Primary Antibodies »

Product Specifications

Immunogen

Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary) Swissprot Not Applicable

Localization

Cell surface. Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands.

Negative Control
Negitve control:Gastric tissue: Normal epithelial and stromal cells should not stain.

Applications for Helicobacter Pylori Antibody (MSVA-466M) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Helicobacter Pylori

This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Alternate Names

H. pylori

Additional Helicobacter Pylori Products

Product Documents for Helicobacter Pylori Antibody (MSVA-466M) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Helicobacter Pylori Antibody (MSVA-466M) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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