Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

Novus Biologicals | Catalog # NBP2-23522

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunoprecipitation, Immunoaffinity Purification

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A71

Format

BSA Free
View all Heparan Sulfate Proteoglycan 2 Primary Antibodies »

Product Specifications

Immunogen

Bovine corneal endothelial cells

Epitope

Epitope is located in domain I

Specificity

Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Applications for Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
ELISA: NBP2-23522 can be used in ELISA. WB: In Western blotting a dilution guideline of 1/100 has proved successful (1,2) IHC: NBP2-23522 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP2-23522 can be used in affinity chromatography to separate recombinant domain I from tissue culture fluid. (1) IP: NBP2-23522 can be used in immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Heparan Sulfate Proteoglycan 2

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

Alternate Names

basement membrane-specific heparan sulfate proteoglycan core protein, endorepellin (domain V region), heparan sulfate proteoglycan 2, HSPG, perlecan, perlecan proteoglycan, PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia), PRCAN, SJA, SJS, SJS1

Gene Symbol

HSPG2

Additional Heparan Sulfate Proteoglycan 2 Products

Product Documents for Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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