HGFR/c-MET Antibody (A9A4-R)

Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198]

Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution.

Lane 1: A549 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Hela cell lysate
Lane 5: LO2 cell lysate
Lane 6: SK-Br-3 cell lysate (Negative)

Lysates/proteins at 30 ug/Lane.

Predicted band size: 156 kDa
Observed band size: 145 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198]

Immunohistochemistry: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32198) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.