![Western Blot: HIPK4 Antibody [NBP3-12399]](https://resources.rndsystems.com/images/products/HIPK4-Antibody-Western-Blot-NBP3-12399-img0006.jpg "Western Blot: HIPK4 Antibody [NBP3-12399]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
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Product Specifications
Immunogen
Synthetic peptide taken within amino acid region 470-550 on human Homeodomain-interacting protein kinase 4.
Reactivity Notes
Baboon (100%).
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for HIPK4 Antibody
Western Blot: HIPK4 Antibody [NBP3-12399]
Western Blot: HIPK4 Antibody [NBP3-12399] - 1) Wild type mouse testis extract 10 uL. ) 2) kO mouse testis extract 10 uL. 3) 3T3 cells overexpressing mouse 2.5 uL. Apparent MW is 72-80 KDa. 1:250 dilution in DilObuffer.![Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]](https://resources.rndsystems.com/images/products/HIPK4-Antibody-Immunohistochemistry-Paraffin-NBP3-12399-img0005.jpg "Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]")
Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]
Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Rat testis. 1:50. IHC blocking buffer. DAB (brown) staining in Hematoxlyin QS (blue) counerstain. 40x magnification.![Western Blot: HIPK4 Antibody [NBP3-12399]](https://resources.rndsystems.com/images/products/HIPK4-Antibody-Western-Blot-NBP3-12399-img0001.jpg "Western Blot: HIPK4 Antibody [NBP3-12399]")
Western Blot: HIPK4 Antibody [NBP3-12399]
Western Blot: HIPK4 Antibody [NBP3-12399] - Lane A: Human over expressing NIH 3T3 cells reduced. Lane B: Mouse over expressing NIH 3T3 cells. Lane C: Mock transfected NIH 3TS3 cells. Dilution 1:500. Total protein per lane 2.5 ug. Apparent MW is 72-80 KDa.![Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]](https://resources.rndsystems.com/images/products/HIPK4-Antibody-Immunohistochemistry-Paraffin-NBP3-12399-img0002.jpg "Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]")
Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]
Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining in Hematoxlyin QS (blue) counerstain. 40x magnification.![Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]](https://resources.rndsystems.com/images/products/HIPK4-Antibody-Immunohistochemistry-Paraffin-NBP3-12399-img0003.jpg "Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]")
Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399]
Immunohistochemistry-Paraffin: HIPK4 Antibody [NBP3-12399] - Baboon kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining in Hematoxlin QS (blue) counterstain. 40x magnification.Applications for HIPK4 Antibody
Application
Recommended Usage
ELISA
1:5000
Immunohistochemistry-Paraffin
1:50-1:150
Immunoprecipitation
1:200
Western Blot
1:2500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: HIPK4
Alternate Names
EC 2.7.11.1, FLJ32818, homeodomain interacting protein kinase 4, homeodomain-interacting protein kinase 4
Gene Symbol
HIPK4
Additional HIPK4 Products
Product Documents for HIPK4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HIPK4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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