Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Novus Biologicals | Catalog # NBP3-50259

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2900
View all Histone Deacetylase 2/HDAC2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Histone Deacetylase 2/HDAC2 (Uniprot: Q92769)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (23GB2900)[NBP3-50259] -

Immunocytochemical staining of HepG2 cells with Histone Deacetylase 2/HDAC2 antibody (1:1,000). Nuclei were stained blue with DAPI; Histone Deacetylase 2/HDAC2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Flow Cytometry: Histone Deacetylase 2/HDAC2 Antibody (23GB2900)[NBP3-50259] -

Flow cytometric analysis of Histone Deacetylase 2/HDAC2expression in HepG2 cells using Histone Deacetylase 2/HDAC2 antibody (1:2,000). Green, isotype control; red, Histone deacetylase 2.
Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (23GB2900)[NBP3-50259] -

Western blotting analysis using Histone Deacetylase 2/HDAC2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (23GB2900)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail