HSD17B4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85297

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (90%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSD17B4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LRFDGRVVLVTGAGAGLGRAYALAFAERGALVVVNDLGGDFKGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSD17B4 Antibody - BSA Free

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining in human liver and pancreas tissues using anti-HSD17B4 antibody. Corresponding HSD17B4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human liver, lung, lymph node and testis using Anti-HSD17B4 antibody NBP1-85297 (A) shows similar protein distribution across tissues to independent antibody NBP1-85296 (B).
Western Blot: HSD17B4 Antibody [NBP1-85297]

Western Blot: HSD17B4 Antibody [NBP1-85297]

Western Blot: HSD17B4 Antibody [NBP1-85297] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody [NBP1-85297]

Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody [NBP1-85297]

Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody [NBP1-85297] - Staining of human cell line U-2 OS shows localization to peroxisomes.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human lung.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human liver shows high expression.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human lymph node.
Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297]

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human testis.

Applications for HSD17B4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSD17B4

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Alternate Names

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, 7-alpha, D-bifunctional protein, EDH17B4, hydroxysteroid (17-beta) dehydrogenase 4, MPF-2, peroxisomal, peroxisomal multifunctional enzyme type 2,17-beta-HSD IV, peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV, short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Gene Symbol

HSD17B4

Additional HSD17B4 Products

Product Documents for HSD17B4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSD17B4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSD17B4 Antibody - BSA Free

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Protocols

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FAQs

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