HSP70/HSPA1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-97872

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Plant, Primate, Sheep, Yeast

Cited:

Avian

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP70/HSPA1A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to a portion of rat Hsp70.

Reactivity Notes

Avian reactivity reported in scientific literature (PMID:31283944).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HSP70/HSPA1A Antibody - BSA Free (NBP1-97872) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-HSP70/HSPA1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HSP70/HSPA1A Antibody - BSA Free

Western Blot: HSP70/HSPA1A Antibody [NBP1-97872]

Western Blot: HSP70/HSPA1A Antibody [NBP1-97872]

Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker, Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate, Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP1-97872]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP1-97872]

Immunohistochemistry-Paraffin: HSP70 / HSC70 Antibody [NBP1-97872] - Analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10ug/ml.

Applications for HSP70/HSPA1A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1:1000
Application Notes
Use in ELISA reported in scientific literature (PMID: 31283944).

Reviewed Applications

Read 1 review rated 5 using NBP1-97872 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

UniProt

Q07439 (Rat)

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP70/HSPA1A Antibody - BSA Free

Customer Reviews for HSP70/HSPA1A Antibody - BSA Free (1)

5 out of 5
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  • HSP70/HSPA1A Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Duck
    Verified Customer | Posted 02/14/2018

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Protocols

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail