HSP70/HSPA1A [ac Lys77] Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-61122

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP70/HSPA1A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Modification

ac Lys77

Localization

Cytoplasm, Nucleus

Specificity

Binds to acetylated lysine 77 on HSP70. Detects 70 kDa band.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A [ac Lys77] Antibody - BSA Free

Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122]

Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122]

Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122]

Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122]

Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Tissue: Colon Cancer. Species: Human. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody (NBP2-61122) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Applications for HSP70/HSPA1A [ac Lys77] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:60

Immunohistochemistry

1:50

Immunohistochemistry-Frozen (Negative)

1:50

Western Blot

1:1000
Application Notes
A 1:1000 dilution of this antibody was sufficient for detection of HSP70 Acetyl Lys77 in 10 ug of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A [ac Lys77] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP70/HSPA1A [ac Lys77] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail