Human ADAM8 aa 498‑653 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB10311AFP488

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 143338
View all ADAM8 Primary Antibodies »

Product Specifications

Specificity

Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498‑653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human ADAM8 aa 498‑653 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

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Spectra Viewer
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Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

References

  1. Yoshiyama, K. et al. (1997) Genomics 41:56.
  2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
  3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
  6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

Long Name

A Disintegrin and Metalloprotease-like Domain 8

Alternate Names

CD156a, MS2

Entrez Gene IDs

101 (Human)

Gene Symbol

ADAM8

UniProt

P78325

Additional ADAM8 Products

Product Documents for Human ADAM8 aa 498‑653 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ADAM8 aa 498‑653 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Metalloproteases and Regulators

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Protocols

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FAQs

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