Human ADAM8 aa 498-653 Antibody (143338)

R&D Systems | Catalog # MAB10311

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, FACS, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 143338
View all ADAM8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain
Asp158-Ser653
Accession # P78325

Specificity

Detects human ADAM8 in direct ELISAs and Western blots. Detects aa 498‑653 of rhADAM8, which corresponds to the cysteine-rich and EGF-like domains. In direct ELISAs, no cross-reactivity with recombinant human (rh) ADAM9, rhADAM10, rhADAM15, rhBACE, or rhTACE is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ADAM8 aa 498-653 Antibody (143338)

Detection of Human ADAM8 antibody by Western Blot.

Detection of Human ADAM8 by Western Blot.

Western blot shows Recombinant Human ADAM8 aa158-653 and Recombinant Human ADAM8 aa 158-497. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ADAM8 Monoclonal Antibody (Catalog # MAB10311) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ADAM8 aa158-653 at approximately 65 kDa (as indicated). The antibody did not detect ADAM8 aa 158-497, indicating it is specific for aa 498-653. This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.

Applications for Human ADAM8 aa 498-653 Antibody (143338)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM8 aa 158-653 see our available Western blot detection antibodies. Does not detect Recombinant Human ADAM8 aa 158-497.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human ADAM8 aa 158-653 under non-reducing conditions only. Does not detect recombinant Human ADAM8 aa 158-497.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824).

References

  1. Yoshiyama, K. et al. (1997) Genomics 41:56.
  2. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227.
  3. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
  4. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
  5. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814.
  6. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.

Long Name

A Disintegrin and Metalloprotease-like Domain 8

Alternate Names

CD156a, MS2

Entrez Gene IDs

101 (Human)

Gene Symbol

ADAM8

UniProt

P78325

Additional ADAM8 Products

Product Documents for Human ADAM8 aa 498-653 Antibody (143338)

Certificate of Analysis

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Product Specific Notices for Human ADAM8 aa 498-653 Antibody (143338)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ADAM8 aa 498-653 Antibody (143338)

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Protocols

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