Human Aggrecan G1-IGD-G2 Domains Antibody (179509)

Detection of Mouse Human Aggrecan G1-IGD-G2 Domains Antibody by Immunocytochemistry/ Immunofluorescence

Olanzapine (Oz) and haloperidol (Hp) modify the density of excitatory and inhibitory synapses to excitatory and inhibitory neurons. (a) The principle of the synapse quantification method is based on the detection of presynaptic and postsynaptic markers. (b) The overlap between presynaptic and postsynaptic fluorescence allows to identify the structurally complete synapses. Neurotransmitter transporter proteins are labeled in red at the presynapse, scaffolding protein is marked green at the postsynapse. (c) Glutamatergic and GABAergic synapses are detected with reference to aggrecan expression in 21 days in vitro (21 DIV) neuronal cultures. The representative 66.5 × 66.5 µm single plane confocal micrographs exemplify the staining patterns of excitatory (colocalization of PSD95 and VGlut1) and inhibitory (colocalization of gephyrin and VGAT) synapses in proximity to inhibitory (aggrecan-positive) and excitatory (aggrecan-negative) neuron somata (See explanation in the text). High-resolution scans were obtained from the regions proximal to neuronal somata. Scale bar, 30 µm. (d) The percent of increase/decrease in synapse density upon treatment with antipsychotics is shown as median (square center), and the inter quartile range (25–75% IQR whiskers). Each data point reflects the quantification of minimum 35 images (66.5 × 66.5 µm area, containing a single cell body of a 21 DIV neuron, exemplified in (c), N = 5. The asterisks indicate significant differences with the corresponding control, based on the Kruskal-Wallis ANOVA test (*p < 0.05; ***p < 0.001). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28912551), licensed under a CC-BY license. Not internally tested by R&D Systems.

Aggrecan in Human Cartilage.

Aggrecan was detected in immersion fixed paraffin-embedded sections of human cartilage using Mouse Anti-Human Aggrecan G1-IGD-G2 Domains Monoclonal Antibody (Catalog # MAB1220) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to chondrocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.