Human alpha 2-Macroglobulin Antibody

R&D Systems | Catalog # AF1938

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all alpha 2-Macroglobulin Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived alpha 2-Macroglobulin

Specificity

Detects human alpha

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human alpha 2-Macroglobulin Antibody

Detection of Human a2-Macroglobulin antibody by Western Blot.

Detection of Human alpha 2‑Macroglobulin by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human a2-Macroglobulin antibody by Simple WesternTM.

Detection of Human alpha 2‑Macroglobulin by Simple WesternTM.

Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for a2-Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
a2-Macroglobulin antibody in human PBMCs by Immunocytochemistry (ICC).

alpha 2‑Macroglobulin in human PBMCs.

a2-Macroglobulin was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human alpha 2-Macroglobulin ELISA Standard Curve

Human alpha 2-Macroglobulin ELISA Standard Curve

Human ?2‑Macroglobulin (Catalog # 1938-PI) was serially diluted and captured by Mouse Anti-Human ?2‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ?2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human alpha 2-Macroglobulin ELISA Standard Curve

Human alpha 2-Macroglobulin ELISA Standard Curve

Human alpha 2‑Macroglobulin (Catalog # 1938-PI) was serially diluted and captured by Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human alpha 2-Macroglobulin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Human alpha 2-Macroglobulin (Catalog # 1938-PI), see our available Western blot detection antibodies

Simple Western

1 µg/mL
Sample: Human serum

Western Blot

0.5 µg/mL
Sample: Human serum

Reviewed Applications

Read 4 reviews rated 4.3 using AF1938 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 2-Macroglobulin

Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.

References

  1. Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
  2. Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Entrez Gene IDs

2 (Human)

Gene Symbol

A2M

Additional alpha 2-Macroglobulin Products

Product Documents for Human alpha 2-Macroglobulin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human alpha 2-Macroglobulin Antibody

For research use only

Citations for Human alpha 2-Macroglobulin Antibody

Customer Reviews for Human alpha 2-Macroglobulin Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 的 4 reviews Showing All
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  • Human alpha 2-Macroglobulin Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human alpha 2-Macroglobulin Antibody AF1938
  • Human alpha 2-Macroglobulin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2018
  • Human alpha 2-Macroglobulin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma and Serum
    Species: Human and Mouse
    Verified Customer | Posted 11/07/2018
  • Human alpha 2-Macroglobulin Antibody
    Name: Carly Garrison
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018

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