Human alpha-Internexin Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB4844R Datasheet / COA / SDS

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FAB4844R-100UG

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Human alpha-Internexin Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ‑Internexin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NF-M or rhVimentin is observed.

Source

Monoclonal Mouse IgG1 Clone # 529724

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Î±-Internexin
Val230-Glu450
Accession # Q16352

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: alpha-Internexin

alpha -Internexin ( alpha -Inx; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -Inx is one of four Class IV neuronal IF proteins. It both self-assembles and complexes with NF-L, H and M in select cortical and cerebellar neurons. alpha -Inx contains one DNA-binding region (aa 10‑92), a poly‑Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -Inx shows more that 96% aa identity with mouse, canine and rat alpha -Inx.

Entrez Gene IDs

9118 (Human); 226180 (Mouse); 24503 (Rat)

Alternate Names

66 kDa neurofilament protein; alphaInternexin; alpha-Internexin; alpha-Inx; FLJ18662; FLJ57501; INA; internexin neuronal intermediate filament protein, alpha; MGC12702; NEF5; neurofilament 5 (66kD); Neurofilament 5; Neurofilament-66; neurofilament-66, tax-binding protein; NF-66; NF-66alpha-internexin; TXBP-1